Antimicrobial capacity against S. mutans biofilms was investigated using a collagen-coated Calgary Biofilm Device (CFU and Live/Dead Confocal). Collagenases from Clostridium histolyticum, Porphyromonas gingivalis, and S. mutants were used to assess anti-collagenolytic activity.
The biocompatibility of both compounds with Human dental pulp stromal Cells (HDPSCs) was investigated in 3 different donors (DREC ethical approval 251121/HA/336). The findings were published in the International Association for Dental Research (IADR).
AA inhibited the growth of S. mutans and V. parvula, as well as partially inhibited bacterial collagenases (>5μg/mL). LDT11 (100μg/mL) inhibited 96% of collagenase activity. AA treatment was associated with odontoblast-like morphology, which was observed after 24h of treatment.
LDT11 at 50 ug/mL concentration had bacteriostatic activity against S. mutans and V. parvula, antimicrobial activity against mature S. mutans biofilms as well as anti-collagenolytic activity against bacterial collagenases. It was biocompatible with HDPSCs, stimulating cells proliferation and differentiation.